APE1 (APEX1) (2-15) Goat Polyclonal Antibody

CAT#: AP23022PU-N

APE1 (APEX1) (2-15) goat polyclonal antibody, Aff - Purified

Product Images

Western Blot Image: Staining (0.5ug/ml) of A431 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected using chemiluminescence.

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  Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunohistochemistry Image: Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 0.2 - 1 µg/ml.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from N Terminus of APE1 / APEX1 - KLH conjugated
• Specificity: This antibody detects APEX1 / REF-1 (N-term).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens apurinic/apyrimidinic endodeoxyribonuclease 1 (APEX1), transcript variant 1
• Database Link:
  Entrez Gene 328 Human
• Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This gene encodes the major AP endonuclease in human cells. Splice variants have been found for this gene; all encode the same protein.
• Synonyms: APE, APEX, APX, HAP1, REF1, APEN

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Base excision repair