KAP1 (TRIM28) (823-835) Goat Polyclonal Antibody

CAT#: AP23026PU-N

KAP1 (TRIM28) (823-835) goat polyclonal antibody, Aff - Purified

Product Images

Western Blot Image: Staining (0.5 ug/ml) of HepG2 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

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  Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunohistochemistry Image: Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA.
  Immunofluorescence.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 0.2 - 1 µg/ml.
• Reactivities: Human, Monkey
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C Terminus of human KAP1 / TRIM28
• Specificity: This antibody detects TIF1-beta / TRIM28 at C-term.
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Immunoaffinity chromatography
• Gene Name: Homo sapiens tripartite motif containing 28 (TRIM28)
• Database Link:
  Entrez Gene 10155 Human
• Background: TIF1beta is a member of the Transcriptional Intermediary Factor 1 (TIF1) subfamily. TIF1beta contains a RING finger, B box, Coiled coil, PHD/TTC, and bromodomain. TIF1beta is a corepressor for Kruppel-associated box (KRAB)-domain-containing zinc finger proteins and plays a critical role in early embryogenesis. TIF1beta acts as a transcriptional mediator by binding liganded nuclear receptors, including retinoic acid (RAR), retinoid X (RXR) and estrogen (ER) receptors. TIF1beta associates with both heterochromatin and euchromatin, causing gene silencing by both HP1 binding and histone deacetylation.
• Synonyms: TIF1 beta, TIF1B, KAP1, KAP-1, KRIP1, KRIP-1, RNF96, RING finger protein 96

Reference Data

• Protein Families: Protein Kinase, Stem cell - Pluripotency, Transcription Factors