GAD65 (GAD2) (515-528) Goat Polyclonal Antibody

CAT#: AP23035PU-N

GAD65 (GAD2) (515-528) goat polyclonal antibody, Aff - Purified

Product Images

Western Blot Image: Antibody (0.1 ug/ml) staining of Human Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Immunohistochemistry Image: Human Brain, cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunohistochemistry Image: Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 1/32000, 0.1 - 0.3 µg/ml.
• Reactivities: Human, Monkey, Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human GAD2
• Specificity: This antibody detects GAD2 / GAD65 (Internal).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glutamate decarboxylase 2 (GAD2), transcript variant 1
• Database Link:
  Entrez Gene 14417 MouseEntrez Gene 613029 MonkeyEntrez Gene 2572 Human
• Background: GAD65 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantibody and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.
• Synonyms: GAD-2, GAD-65, Glutamate decarboxylase 2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus