IDE (914-924) Goat Polyclonal Antibody

CAT#: AP23052PU-N

IDE (914-924) goat polyclonal antibody, Aff - Purified

Product Images

Western Blot Image: Antibody (0.1 ug/ml) staining of K562 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Immunohistochemistry Image: Human Breast: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunohistochemistry Image: Human Lung: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot: 0.1 - 0.3 µg/ml.
• Reactivities: Bat, Canine, Equine, Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human IDE
• Specificity: This antibody detects Insulin-degrading enzyme (Internal).
  It is expected to recognize both reported isoforms (NP_004960.1 and NP_001159418.1).
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens insulin degrading enzyme (IDE), transcript variant 1
• Database Link:
  Entrez Gene 3416 Human
• Background: Insulin Degrading Enzyme (IDE) encodes a zinc metallopeptidase that degrades intracellular insulin, and thereby terminates insulins activity, as well as participating in intercellular peptide signalling by degrading diverse peptides such as glucagon, amylin, bradykinin, and kallidin. The preferential affinity of IDE for insulin results in insulin-mediated inhibition of the degradation of other peptides such as beta-amyloid. Deficiencies in IDE's function are associated with Alzheimer's disease and type 2 diabetes mellitus but mutations in this gene have not been shown to be causitive for these diseases. This protein localizes primarily to the cytoplasm but in some cell types localizes to the extracellular space, cell membrane, peroxisome, and mitochondrion.
• Synonyms: IDE, EC=3.4.24.56, Insulin protease, Insulinase, Insulysin, Abeta-degrading protease

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Alzheimer's disease