Frizzled 2 (FZD2) Goat Polyclonal Antibody

CAT#: AP23061PU-N

Frizzled 2 (FZD2) goat polyclonal antibody, Purified

Product Images

Immunohistochemistry: Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunohistochemistry: Human Thyroid: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: ELISA: 1/64000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
• Reactivities: Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the internal region of the human protein sequence according to NP_001457.1
• Specificity: This antibody detects FZD2 / Frizzled-2 (Internal).
• Formulation: Tris saline, pH 7.3 containing 0.02% sodium azide as preservative and 0.5% BSA as stabilizer
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens frizzled class receptor 2 (FZD2)
• Database Link:
  Entrez Gene 57265 MouseEntrez Gene 64512 RatEntrez Gene 2535 Human
• Background: FZD2 is a Frizzled Receptor that mediates Wnt signaling, which is required for proper axis development in the embryo. FZD2 is developmentally regulated. Studies of rat FZD2 indicate that the protein signals through a calcium-releasing pathway and that FDZ2 expression modulates protein kinase C (PKC) localization and stimulates PKC activity in vitro. Increased levels of FZD2 mRNA have been observed in primary gastric cancer and in head and neck squamous carcinoma and gastric cancer cell lines.
• Synonyms: Fz-2, hFz2, FzE2
• Note: Western Blot: Preliminary experiments gave an approx. 23kD band in Human Colon, Heart and Ovary lysates after 0.1 µg/ml antibody staining.
  Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 63.6kD according to NP_001457.1.
  The 23kD band was successfully blocked by incubation with the immunizing peptide.
  We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Basal cell carcinoma, Colorectal cancer, Melanogenesis, Pathways in cancer, Wnt signaling pathway