Oct4 (POU5F1) (6-152) Rabbit Polyclonal Antibody

CAT#: AP23147PU-N

Oct4 (POU5F1) (6-152) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (20 ug ). A: HeLa nucleus. 10% SDS PAGE. POU5F1 antibody diluted at 1:1000.

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  Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using OCT3/4 antibody at 1:100 dilution.

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  Immunofluorescence analysis of paraformaldehyde-fixed human embryonic stem cell, using OCT3/4 antibody at 1:100 dilution, costained with DAPI.

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  Flow cytometry on human embryonic stem cells, staining with OCT3/4 antibody at 1:50 dilution (green) or rabbit IgG (black).

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Specifications

Product Data

• Applications: FC, IF, IHC, WB
• Recommended Dilution: Flow Cytometry.
  Immunocytochemistry: 1/100 - 1/200.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 6 and 152 of OCT3/4
• Specificity: This antibody detects OCT3/4 at aa 6-152.
• Formulation: PBS, pH 7, 10% Glycerol, 0.01% Thimerosal
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store (in aliquots) at -20 °C. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 5460 Human
• Background: POU5F1 / OCT4 is a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms.
• Synonyms: POU5F1, OCT3, OCT4, OTF3, POU domain, class 5, transcription factor 1, Octamer-binding transcription factor 3, Oct-3, Oct-4