HIF-1 alpha (HIF1A) Rabbit Polyclonal Antibody

CAT#: AP23253PU-N

HIF-1 alpha (HIF1A) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat brain tissue lysis using HIF-1alpha antibody

• 

  Immunohistochemical analysis of paraffin embedded Mammary cancer sections, staining HIF-1alpha in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western blot: At 1-2 μg/ml with the appropriate system to detect HIF-1α in cells and tissues.
  Immunohistochemistry on Paraffin Section: At 1-2 μg/ml to detect HIF-1α in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A peptide mapping at the N-terminus of HIF-1α of Human origin, different from the related Rat and Mouse sequences by two sequences.
• Specificity: This antibody detects HIF1A / HIF1 alpha.
• Formulation: 50% Glycerol, 0.9 mg NaCl, 0.2 mg Na₂HPO₄
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 1.2% Sodium Acetate or neutral PBS. If 0.5 ml of PBS is used, the antibody concentration will be 200 μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens hypoxia inducible factor 1 alpha subunit (HIF1A), transcript variant 1
• Database Link:
  Entrez Gene 15251 MouseEntrez Gene 29560 RatEntrez Gene 3091 Human
• Background: HIF-1α (Hypoxia-inducible factor 1α,HIF1A) is a transcription factor that mediates cellular and systemic homeostatic responses to reduced O2 availability in mammals, including angiogenesis, erythropoiesis and glycolysis. This gene was mapped to 14q21-q24. HIF-1α transactivate genes required for energy metabolism and tissue perfusion and is necessary for embryonic development and tumor explant growth. HIF-1alpha is over expressed during carcinogenesis, myocardial infarction and wound healing.It is crucial for the cellular response to hypoxia and is frequently over expressed in human cancers,resulting in the activation of genes essential for cell survival. HIF-1α regulates the survival and function in the inflammatory microenvironment directly. It is a transcription factor that plays a pivotal role in cellular adaptation to changes in oxygen availability.
• Synonyms: HIF-1 alpha, Hypoxia-inducible factor 1 alpha, ARNT-interacting protein, Member of PAS protein 1, Basic-helix-loop-helix-PAS protein MOP1, BHLHE78

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: mTOR signaling pathway, Pathways in cancer, Renal cell carcinoma