DUT Rabbit Polyclonal Antibody

CAT#: AP23256PU-N

DUT rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical analysis of paraffin embedded rat tissue sections (cardiac muscle), staining DUTPase in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, IP, WB
• Recommended Dilution: Western blot: At 0.5-1μg/ml with the appropriate system to detect dUTPase in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect dUTPase in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
  Immunoprecipitation.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence mapping at the middle region of human dUTPase
• Specificity: This antibody detects DUT in the middle. No cross reactivity with other roteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg himerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens deoxyuridine triphosphatase (DUT), transcript variant 1
• Database Link:
  Entrez Gene 110074 MouseEntrez Gene 497778 RatEntrez Gene 1854 Human
• Background: Deoxyuridine triphosphate nucleotidohydrolase (dUTPase) is responsible for maintaining low ntracellular levels of dUTP, thus preventing the incorporation of dUTP into DNA. DUTPase ctivity/expression can be down-regulated using siRNA specifically targeted to dUTPase mRNA and UTPase plays a role in DNA nucleotide metabolism. This protein, present predominantly in the ytoplasm, contains 252 amino acids with a Mr of 26,704. It exhibits 35% identity with the E. coli UTPase and 53% identity with the Saccharomyces cerevisiae enzyme. The nuclear and mitochondrial orms of dUTPase are encoded by the same gene with isoform-specific transcripts arising through the se of alternative 5-prime exons. Human dUTPase exhibits 92% identity with rat. Moreover, this nzyme has profound effects on the efficacy of agents that target thymidylate biosynthesis.
• Synonyms: dUTPase, dUTP pyrophosphatase

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pyrimidine metabolism