FLIP (CFLAR) (Long Form) Rabbit Polyclonal Antibody

CAT#: AP23261PU-N

FLIP (CFLAR) (Long Form) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemical FLIPL antibody staining of Human Mammary Cancer Paraffin Sections.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: Use at 1-2 μg/ml with the appropriate system to detect FLIP-L in cells and tissues.
  Immunohistochemistry on Paraffin Sections: Use at 1-2 μg/ml to detect FLIP-L in Formalin Fixed and Paraffin Embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence mapping at the C-terminal of Human FLIP-L, identical to the related Mouse sequence.
• Specificity: Recognizes FLIP-L
  No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal and 0.05mg Sodium Azide
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore with 0.2 ml of distilled water to yield a concentration of 0.5 mg/ml.
• Concentration: 0.5 mg/ml (after reconstitution)
• Purification: Immunoaffinity Chromatography
• Gene Name: Homo sapiens CASP8 and FADD like apoptosis regulator (CFLAR), transcript variant 2
• Database Link:
  Entrez Gene 12633 MouseEntrez Gene 117279 RatEntrez Gene 8837 Human
• Background: Irmler et al. (1997) isolated human cDNAs encoding 2 isoforms of FLIP. FLIP(L), the longer isoform, contains 2 DEDs and a caspase-like domain, and FLIP(S), the shorter isoform, contains only the 2 DEDs followed by a C-terminal extension of approximately 50 amino acids. High levels of FLIP(L) protein were detected in melanoma cell lines and malignant melanoma tumors. FLIP may be implicated in tissue homeostasis as an important regulator of apoptosis.
• Synonyms: c-FLIP, CLARP, MRIT, CASH, FLAME-1, CASP8AP1, Usurpin

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Apoptosis