BNIP3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP23270PU-N

BNIP3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat thymus tissue lysis using NIP3 antibody

• 

  Immunohistochemical analysis of paraffin embedded cancer sections, staining in nucleus, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: At 1μg/ml with the appropriate system to detect NIP3 in cells and tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence near the C-terminal of human NIP3
• Specificity: This antibody detects NIP3 near C-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Gene Name: Homo sapiens BCL2 interacting protein 3 (BNIP3)
• Database Link:
  Entrez Gene 12176 MouseEntrez Gene 84480 RatEntrez Gene 664 Human
• Background: The Bcl-2 nineteen kilodalton interacting protein 3 (BNIP3 or NIP3), is a hypoxia-inducible proapoptotic member of the Bcl-2 family that induces cell death by associating with the mitochondria. BNIP3,expressed in skeletal muscle and in the brain at low levels, is primarily localized to the nucleus of glial cells of the normal human brain, as well as in the malignant glioma cell line U251. BNIP3 expression in the cytoplasm increases and localizes with the mitochondria, contributing to induction of cell death.Cellular protein BNIP3 interacts with E1B-19K, BCL-2, BCL-xL, and EBV-BHRF1. BNIP3 contains Bcl-2 homology 3 (BH3) domain and COOH-terminal transmembrane (TM) domain. The BH3 domain of BNIP3 mediates Bcl-2/Bcl-X(L) heterodimerization and confers pro-apoptotic activity; whereas the TM domain is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting.
• Synonyms: BNIP3

Reference Data

• Protein Families: Druggable Genome, Transmembrane