ROCK2 (N-term) Rabbit Polyclonal Antibody

CAT#: AP23285PU-N

ROCK2 (N-term) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1 µg/ml.
  Immunohistochemistry on paraffin sections: 2 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence at the N-terminal of human ROCK2
• Specificity: This antibody detects ROCK2 (N-term).
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg
  Thimerosal, 0.05mg NaN3.
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Gene Name: Homo sapiens Rho associated coiled-coil containing protein kinase 2 (ROCK2), transcript variant 1
• Database Link:
  Entrez Gene 19878 MouseEntrez Gene 25537 RatEntrez Gene 9475 Human
• Background: Rho-associated kinase (ROCK), including the ROCK-I and ROCK-II isoforms, is a protein kinase involved in signaling from Rho to actin cytoskeleton. Serine/threonine kinase ROCK II/Rho kinase, which is an isozyme of ROCK I, is one of the targets for the small GTPase Rho. ROCK II regulates the formation of actin stress fibers and focal adhesions, cytokinesis, smooth muscle contraction, and the activation of c-fos serum response element. Sequencing analysis has shown that human ROCK II contains 1388 amino acid residues with a calculated molecular mass of approximately 161 kDa.
  Fluorescence in situ hybridization analysis showed that the human ROCK II gene is located on chromosome 2p24.1 Thumkeo et al. (2003) concluded that ROCK-II is essential in inhibiting blood coagulation and maintaining blood flow in the endothelium-free labyrinth layer and that loss of ROCK-II leads to thrombus formation, placental dysfunction, intrauterine growth retardation, and fetal death.2
• Synonyms: ROCK-2, p164ROCK-2, KIAA0619

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, TGF-beta signaling pathway, Vascular smooth muscle contraction, Wnt signaling pathway