GJB2 Rabbit Polyclonal Antibody

CAT#: AP23307PU-N

GJB2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Rat liver sections using Connexin 26 antibody

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect Connexin 26 in cells and tissues.
  Immunocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide mapping at the middle region of rat Connexin 26
• Specificity: This antibody detects GJB2 / Cx26 (mid region). No cross reactivity with other proteins.
• Formulation: 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens gap junction protein beta 2 (GJB2)
• Database Link:
  Entrez Gene 14619 MouseEntrez Gene 394266 RatEntrez Gene 2706 Human
• Background: Connexin26(CX26), also known as GAP junction protein, beta2, GJB2. Gap junctions were first characterized by electron microscopy as regionally specialized structures on plasma membranes of contacting adherent cells. These structures were shown to consist of cell-to-cell channels. Proteins,called connexins, purified from fractions of enriched gap junctions from different tissues differ. The 3-prime untranslated region of the CX26 transcript contains a putative mRNA instability sequence. The deduced 226-amino acid protein has a calculated molecular mass of about 26 kD. CX26 shares 92.5% identity with rat Cx26. connexin 26 (GJB2) is assigned to human chromosome 13q11-q12. Connexin 26 regulates epidermal barrier and wound remodeling and promotes psoriasiform response. Connexin 26 gene (GJB2) mutation modulates the severity of hearing loss associated with the 1555A-G mitochondrial mutation.
• Synonyms: Gap junction beta-2 protein, Connexin-26

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane