Estrogen Receptor beta (ESR2) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23349PU-N

Estrogen Receptor beta (ESR2) (N-term) rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: At 0.5-1μg/ml with the appropriate system to detect ERβ in cells and tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide mapping at the N-terminal of human ERβ
• Specificity: This antibody detects Estrogen receptor beta.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens estrogen receptor 2 (ER beta) (ESR2), transcript variant b
• Database Link:
  Entrez Gene 13983 MouseEntrez Gene 25149 RatEntrez Gene 2100 Human
• Background: Estrogen receptor-beta, referred to as ESR2, is a member of the superfamily of nuclear receptors,which can transduce extracellular signals into transcriptional responses. This gene is mapped to 14q and comprises 8 exons spanning approximately 40 kb. ESR2 is expressed in multiple tissues, including developing spermatids of the testis and in ovarian granulosa cells. ESR-beta is homologous to the previously identified ESR-alpha and has an overlapping but nonidentical tissue distribution. The DNA-binding domain of ESR-beta is 96% conserved compared to ESR, and the ligand-binding domain shows 58% conserved residues. ESR-beta is expressed in human thymus, spleen, ovary, and testis. Rat ESR-beta is expressed in rat prostate and ovary and is homologous to rat ESR (95% conserved DNA-binding domain; 55% conserved ligand-binding domain). ESR2 mRNA was coexpressed with ESR1 and its splice variants in 60% of prolactinomas, 100% of mixed growth hormone /prolactin tumors, and 29% of gonadotroph tumors. ESR2 gene expression was not limited to ESR1-positive tumor subtypes, however, and was also found in 100% of null cell tumors, 80% of somatotroph tumors, and 60% of corticotroph tumors.
• Synonyms: ER-beta,ESR2, ESTRB, NR3A2

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors