PMVK Rabbit Polyclonal Antibody

CAT#: AP23367PU-N

PMVK rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of cardiac muscle tissue lysis using PMKV antibody

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  Immunohistochemical analysis of paraffin embedded rat skeletal muscle sections, staining PMVK in cytoplasm, DAB chromeric reaction

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: Use at 0.1-0.5 μg/ml with the appropriate system to detect PMVK in cells and tissues.
  Imunohistochemistry on Paraffin Sections: Use at 0.5-1 μg/ml to detect PMVK in formalin fixed and paraffin embedded tissues.
  Heat Antigen Retrieval isn recommended.
  
  
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence mapping at the middle region of Human PMVK
• Specificity: This antibody detects PMVK (mid region).
  No cross reactivity with other proteins.
• Formulation: 0.2 mg Na2HPO4, 0.9 mg NaCl
  State: Aff - Purified
  State: Lyophilized purified Ig fraction
  Stabilizer: 5 mg BSA
  Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
• Reconstitution Method: 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Purification: Immunoaffinity Chromatography
• Gene Name: Homo sapiens phosphomevalonate kinase (PMVK), transcript variant 1
• Database Link:
  Entrez Gene 68603 MouseEntrez Gene 10654 Human
• Background: PMVK (phosphomevalonate kinase) is a cytosolic enzyme that catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate. It is mapped to chromosome 1p13-1q22-23 and spans more than 8.4 kb in the human genome. PMVK is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle. It was expressed highly in heart, liver, skeletal muscle, kidney and pancreas and slightly lower in brain, placenta, and lung. And PMKase gene expression is subject to regulation by sterol at the level of transcription. It is a single copy gene covering less than 15 kb in the human genome. The human PMKase amino acid sequence contains a consensus peroxisomal targeting sequence (PTS-1), Ser-Arg-Leu, at the C terminus of the protein.
• Synonyms: Phosphomevalonate kinase, PMKase, PMKI, HUMPMKI

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Terpenoid backbone biosynthesis