PLGF (PGF) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23368PU-N

PLGF (PGF) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of human placenta tissue lysis using PLGF antibody

• 

  Immunohistochemical analysis of paraffin embedded human placenta sections; staining in cytoplysm; DAB chromogenic reaction

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect PLGF in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect PLGF in formalin fixed and paraffin embedded tissues.
  Immunocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence mapping near the N-terminal of human PLGF
• Specificity: This antibody detects Placenta growth factor / PGF (N-term). No cross reactivity with other proteins.
• Formulation: 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the
  antibody concentration will be 100μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens placental growth factor (PGF), transcript variant 1
• Database Link:
  Entrez Gene 18654 MouseEntrez Gene 94203 RatEntrez Gene 5228 Human
• Background: Placental growth factor (PGF, also known as PLGF) codes for an angiogenic factor expressed in placental tissue that is similar to vascular permeability factor/vascular endothelial growth factor (VPF/VEGF). It is a glycosylated dimeric secreted protein able to stimulate endothelial cell growth in vitro. PGF is located on chromosome 14 and has been conserved in evolution. It belongs to the family of vascular endothelial growth factors (VEGFs) and binds to the flt-1 VEGF receptor. PLGF-2, which is a PLGF isoform, binds neuropilin-1 and 2 in a heparin-dependent manner. PGF regulates inter-and intra molecular cross talk between the VEGF RTKs Flt1 and Flk1 and stimulates the phosphorylation of specific Flt1 tyrosine residues and the expression of distinct downstream target genes. Moreover, it plays an important role in pathological angiogenic events and with exerting its biological activities through binding to VEGFR1.
• Synonyms: PGFL, PLGF, PlGF

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma