SKP2 (N-term) Rabbit Polyclonal Antibody

CAT#: AP23375PU-N

SKP2 (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

SKP2 Polyclonal Antibody

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  SKP2 Polyclonal Antibody

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 1μg/ml with the appropriate system to detect SKP2 in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect SKP2 in formalin fixed and paraffin embedded tissues.
  Immunocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: synthetic peptide corresponding to a sequence at the N-terminal of human SKP2
• Specificity: This antibody detects SKP2 / FBXL1 (N-term). No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens S-phase kinase-associated protein 2, E3 ubiquitin protein ligase (SKP2), transcript variant 1
• Database Link:
  Entrez Gene 6502 Human
• Background: The F box protein Skp2 (S-phase kinase-associated protein 2) is oncogenic, and its frequent amplification and overexpression correlate with the grade of malignancy of certain tumors. Skp2 controls p300-p53 signaling pathways in cancer cells, making it a potential molecular target for cancer therapy. This gene positively regulates the G(1)-S transition by controlling the stability of several G(1) regulators, such as the cell cycle inhibitor p27. This study provides evidence of a role for an F-box protein in oncogenesis and establishes SKP2 as a protooncogene causally involved in the pathogenesis of lymphomas.
• Synonyms: FBL1; FBXL1; FLB1; MGC1366; p45skp2

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, Cell cycle, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Small cell lung cancer, Ubiquitin mediated proteolysis