CCL28 Rabbit Polyclonal Antibody

CAT#: AP23432BT-S

CCL28 rabbit polyclonal antibody, Aff - Purified


USD 260.00

2 Weeks*

Size
    • 25 ug

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Specifications

Product Data
Applications ELISA, WB
Recommended Dilution Direct ELISA: To detect Human MEC by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Human MEC.
Sandwich ELISA: To detect Human MEC by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated, in conjunction with Purified Anti-Human Visfatin (AP23432PU-N or AP23432PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MEC.
Western Blot: To detect Human MEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivities Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure recombinant Human MEC
Specificity Recognizes MEC.
Formulation PBS, pH 7.2 without preservatives
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography
Gene Name Homo sapiens C-C motif chemokine ligand 28 (CCL28), transcript variant 1
Background CCL28, also known as chemokine (C-C motif) ligand 28 or mucosae-associated epithelial chemokine (MEC), belongs to the subfamily of small cytokine CC proteins. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.
Synonyms C-C motif chemokine 28, SCYA28
Reference Data
Protein Families Druggable Genome, Secreted Protein
Protein Pathways Chemokine signaling pathway, Cytokine-cytokine receptor interaction

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.