CCL28 Rabbit Polyclonal Antibody

CAT#: AP23432PU-S

CCL28 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot (Reduced) using MEC / CCL28 Antibody Cat.-No AP23432PU

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  Western Blot (Unreduced) using MEC / CCL28 Antibody Cat.-No AP23432PU

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  Sandwich ELISA using MEC / CCL28 Antibody Cat.-No AP23432PU

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  Immunofluorescence: This antibody stained CACO-2 cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Sandwich ELISA: To detect Human MEC by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MEC (Cat.-No AP23432BT-N or AP23432BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MEC.
  Western Blot: To detect Human MEC by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MEC is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunofluorescence: This antibody (Lots 0211M242Rb and 0811M242Rb) stained CACO-2 cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure E.coli derived recombinant Human MEC (Cat.-No PA246)
• Specificity: Recognizes Human MEC. Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Human MEC matrix.
• Gene Name: Homo sapiens C-C motif chemokine ligand 28 (CCL28), transcript variant 1
• Database Link:
  Entrez Gene 56477 Human
• Background: CCL28, also known as chemokine (C-C motif) ligand 28 or mucosae-associated epithelial chemokine (MEC), belongs to the subfamily of small cytokine CC proteins. CCL28 is expressed by columnar epithelial cells in the gut, lung, breast and the salivary glands and drives the mucosal homing of T and B lymphocytes that express CCR10, and the migration of eosinophils expressing CCR3. This chemokine is constitutively expressed in the colon, but its levels can be increased by pro-inflammatory cytokines and certain bacterial products implying a role in effector cell recruitment to sites of epithelial injury.
• Synonyms: C-C motif chemokine 28, SCYA28

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction