Col7a1 (NC1 FN-III-like Dom 7+8) Rabbit Polyclonal Antibody

CAT#: AP23438PU-N

Col7a1 (NC1 FN-III-like Dom 7+8) rabbit polyclonal antibody, Purified

Product Images

Immunofluorescence of Collagen VII staining of Cryosections of Murine Skin. The section was incubated with AP23438PU followed by an appropriate secondary antibody coupled to Fluorescein. This antibody marks the dermal-epidermal junction (DEJ). See Sesarman et al. (2008) J. Mol. Med 86 (8): 951-959.

Specifications

Product Data

• Applications: ELISA, IF, IHC
• Recommended Dilution: ELISA.
  Immunofluorescence.
  Immunohistochemistry on Cryosections (Ref.3)
  Induction of Experiemental EBA in Mice (Ref.2,3).
• Reactivities: Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant Murine Collagen type VII (aa 757-967) GST fusion protein.
• Specificity: This antibody recognizes Murine 290 kDa type VII Collagen (epidermal basement membrane / anchoring fibril component).
• Formulation: PBS, pH 7.2
  State: Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore in aqua bidest to 1 mg/ml.
• Purification: Protein G Chromatography
• Gene Name: Mus musculus collagen, type VII, alpha 1 (Col7a1)
• Database Link:
  Entrez Gene 12836 Mouse
• Background: Type VII collagen fibrils are composed of three identical alpha collagen chains. It's location is restricted to the basement zone beneath stratified squamous epithelia. It functions as an anchoring fibril between the external epithelia and the underlying stroma. Mutations in the collagen V11 gene are associated with all forms of dystrophic epidermolysis bullosa.
• Synonyms: COL7A1, Long-chain collagen, LC collagen