14-3-3 beta (YWHAB) (1-141) Rabbit Polyclonal Antibody

CAT#: AP23582PU-N

14-3-3 beta (YWHAB) (1-141) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (30 ug of whole cell lysate). Lane A: A431. Lane B: H1299. Lane C: Hela. Lane D: Hep G2. Lane E: Molt-4. Lane F: Raji. 12% SDS PAGE. YWHAB antibody diluted at 1:10000.

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  Human Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Skin: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using 14-3-3 beta antibody at 1/200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/5000 - 1/20000.
• Reactivities: Human, Zebrafish
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 141 of 14-3-3 beta
• Specificity: This antibody detects 14-3-3 beta at 1-141.
• Formulation: 0.1M Tris-glycine, pH 7, 10% Glycerol, 0.01% Thimerosal
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta (YWHAB), transcript variant 1
• Database Link:
  Entrez Gene 54401 MouseEntrez Gene 56011 RatEntrez Gene 7529 Human
• Background: This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
• Synonyms: YWHAB, KCIP-1, Protein 1054

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle, Neurotrophin signaling pathway, Oocyte meiosis