Gelsolin (GSN) (161-369) Rabbit Polyclonal Antibody

CAT#: AP23589PU-N

Gelsolin (GSN) (161-369) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (30 ug of whole cell lysate). A: H1299. B: Hela. 7.5% SDS PAGE. GSN antibody diluted at 1/1000.

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  Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of methanol-fixed HeLa, using Gelsolin antibody at 1/200 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry: 1/100 - 1/200.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 161 and 369 of Gelsolin.
• Specificity: This antibody reacts to Gelsolin precursor/Actin-depolymerizing factor (GSN/ADF) (Brevin) (AGEL) at aa 161-369.
• Formulation: 0.1 M Tris-glycine, pH 7.0 containing 20% Glycerol as stabilizer and 0.01% Thimerosal as preservative
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens gelsolin (GSN), transcript variant 1
• Database Link:
  Entrez Gene 227753 MouseEntrez Gene 296654 RatEntrez Gene 2934 Human
• Background: Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
• Synonyms: Brevin, AGEL, GSN

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton