Isocitrate dehydrogenase (IDH1) (30-307) Rabbit Polyclonal Antibody

CAT#: AP23607PU-N

Isocitrate dehydrogenase (IDH1) (30-307) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (30 ug whole cell lysate). A:A431. B:Raji. 10% SDS PAGE. IDH1 antibody diluted at 1/1000

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  Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 30 and 307 of Human isocitrate dehydrogenase
• Specificity: This antibody reacts to human Putative Cytosolic Nadp-dependent Isocitrate Dehydrogenase (IDH1) at aa 30-307.
• Formulation: 0.1 M Tris-glycine, pH 7.0 containing 10% Glycerol and 0.01% Thimerosal
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens isocitrate dehydrogenase 1 (NADP+), soluble (IDH1), transcript variant 1
• Database Link:
  Entrez Gene 15926 MouseEntrez Gene 24479 RatEntrez Gene 3417 Human
• Background: Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic.
• Synonyms: PICD, Cytosolic NADP-isocitrate dehydrogenase, ICDH, IDP, Oxalosuccinate decarboxylase

Reference Data

• Protein Pathways: Citrate cycle (TCA cycle), Glutathione metabolism, Metabolic pathways