DNase I (DNASE1) (1-252) Rabbit Polyclonal Antibody

CAT#: AP23615PU-N

DNase I (DNASE1) (1-252) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (30 ug of whole cell lysate). A: MOLT4. 10% SDS PAGE. DNASE1 antibody diluted at 1/1000

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  Human Brain, Cerebellum: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry: 1/100 - 1/1000.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 252 of DNase I.
• Specificity: This antibody recognizes Deoxyribonuclease I (DNASE1) at aa 1-252.
• Formulation: 0.1 M Tris-glycine, pH 7.0 containing 10% Glycerol and 0.01% Thimerosal
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens deoxyribonuclease 1 (DNASE1)
• Database Link:
  Entrez Gene 1773 Human
• Background: Deoxyribonuclease I (EC 3.1.21.1) gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized.
  DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.
• Synonyms: DNASE1, DNL1, DRNI, Deoxyribonuclease I, DNase I

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane