ACTN3 (N-term) Rabbit Polyclonal Antibody

CAT#: AP23644PU-N

ACTN3 (N-term) rabbit polyclonal antibody

Product Images

Western blot analysis of extracts from HT-29 cells, using ACTN3 Antibody. The lane on the right is treated with the synthesized peptide.

• 

  Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/20000.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: ACTN3 antibody was raised against synthetic peptide - KLH conjugated
• Specificity: This antibody reacts to the N-term of Alpha-actinin 3, Skeletal Muscle Isoform (ACTN3).
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM sodium chloride, 0.02% sodium azide as preservative and 50% glycerol as stabilizer
  State: Liquid purfied Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens actinin alpha 3 (gene/pseudogene) (ACTN3), transcript variant 1, coding
• Database Link:
  Entrez Gene 11474 MouseEntrez Gene 171009 RatEntrez Gene 89 Human
• Background: Alpha-actinin is an actin-binding protein with multiple roles in different cell types. ACTN3 expression is limited to skeletal muscle. It is localized to the Z-disc and analogous dense bodies, where it helps to anchor the myofibrillar actin filaments.
• Synonyms: Alpha actinin skeletal muscle isoform 3

Reference Data

• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction