PPPDE1 (DESI2) Goat Polyclonal Antibody

CAT#: AP23656PU-N

PPPDE1 (DESI2) goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human PPPDE1 with C-terminal MYC tag probed with (0.5 ug/ml) in Lane A and probed with anti-MYC Tag (1/5000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western Blot: 0.5-1 µg/ml. In transfected HEK293 transiently expressing full-length Human PPPDE1 (myc and DYKDDDDK tagged), a band of approx. 30kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml in Paraffin embedded Human Skin.
  Purchase the +ve control! The transfected lysate used to QC this product is available for sale.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_057160.2.
• Specificity: Recognizes PPPDE1 / PNAS4.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Database Link:
  Entrez Gene 51029 Human
• Synonyms: C1orf121, FAM152A, CGI-146, PNAS-4, PNAS4
• Note: The positive Control is also available