GLUD1 (+Glutamate dehydrogenase 2) Goat Polyclonal Antibody

CAT#: AP23724PU-N

GLUD1 (+Glutamate dehydrogenase 2) goat polyclonal antibody, Aff - Purified

Product Images

(0.3 ug/ml) staining of Mouse Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  (3.8 ug/ml) staining of paraffin embedded Human Cerebral Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/32000 (Detection Limit).
  Western blot: 0.3-1 µg/ml. Approx 55kDa band observed in Human Brain (Cerebellum) and Mouse Brain lysates and in Human, Mouse and Rat Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1, of 61.3kDa according to Mouse NP_032159.1 and of 61.4kDa according to Rat NP_036702.1).
  Immunofluorescence: Customer found particulate cytoplasm staining in HeLa.
  Immunohistochemistry on Paraffin Sections: 3-5 µg/ml. In paraffin embedded Human Cerebral Cortex shows mitochondrial staining in neuronal cell bodies and axons.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_005262.1; NP_036216.2.
• Specificity: This antibody recognizes Glutamate Dehydrogenase.
  This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glutamate dehydrogenase 1 (GLUD1), transcript variant 1
• Database Link:
  Entrez Gene 14661 MouseEntrez Gene 24399 RatEntrez Gene 2746 Human
• Synonyms: GLUD1, GLUD, GDH1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, D-Glutamine and D-glutamate metabolism, Metabolic pathways, Nitrogen metabolism