Csf2 Rabbit Polyclonal Antibody

CAT#: AP26027PU-N

Csf2 rabbit polyclonal antibody, Purified

Product Images

Western Analysis of anti-Mouse GM-CSF Antibody. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions. Lane 1: MWM (kDa); lane 2: rm GM-CSF; lane 3: rh GM-CSF.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 2-5 μg/ml (Less than 1% cross-reactivity with recombinant Human GM-CSF under non-reducing conditions is observed).
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (>95%) recombinant mouse GM-CSF (Ala18-Lys141) derived from E. coli
• Specificity: This antibody detects Mouse GM-CSF.
  Other species not tested.
• Formulation: PBS, pH 7.2
  State: Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein A Chromatography
• Gene Name: Mus musculus colony stimulating factor 2 (granulocyte-macrophage) (Csf2)
• Database Link:
  Entrez Gene 12981 Mouse
• Background: GM-CSF was initially characterized as a factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is also a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. GM-CSF is produced by a number of different cell types (including T cells, B cells, macrophages, mast cells, endothelial cells, fibroblasts, and adipocytes) in response to cytokine or inflammatory stimuli. On mature hematopoietic cells, GM-CSF is a survival factor for and activates the effector functions of granulocytes, monocytes/macrophages, and eosinophils. GM-CSF promotes a Th1 biased immune response, angiogenesis, allergic inflammation, and the development of autoimmunity. It shows clinical effectiveness in ameliorating chemotherapy-induced neutropenia, and GM-CSF transfected tumor cells are utilized as cancer vaccines.
  The 22 kDa glycosylated GM-CSF, similar to IL3 and IL5, is a cytokine with a core of four bundled α-helices. Mature mouse GM-CSF shares 49-54% amino acid sequence identity with canine, feline, human, and porcine GM-CSF and 69% with rat GMCSF.
  GM-CSF exerts its biological effects through a heterodimeric receptor complex composed of GM-CSF Rα/CD116 and the signal transducing common β chain (CD131) which is also a component of the high affinity receptors for IL3 and IL5. In addition, GM-CSF binds a naturally occurring soluble form of GM-CSF Rα. The activity of GM-CSF is species specific between human and mouse. Mouse GM-CSF is only weakly active on rat cells, although rat GM-CSF is fully active on mouse cells.
• Synonyms: CSF2, GMCSF, Sargramostim, Molgramostin