NANOG (137-141) Rabbit Polyclonal Antibody

CAT#: AP26063PU-S

NANOG (137-141) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of extracts from human Umbilical cord mesenchymal stem cell using Nanog Antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide sequence around aa.137~141 derived from Nanog
• Specificity: This antibody detects endogenous level of total Nanog protein.
• Formulation: Phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.4, 150mM NaCl, 0.02% Sodium Azide and 50% Glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific peptide
• Predicted Protein Size: 42 kDa
• Gene Name: Homo sapiens Nanog homeobox (NANOG), transcript variant 1
• Database Link:
  Entrez Gene 71950 MouseEntrez Gene 79923 Human
• Background: Transcription regulator involved in inner cell mass and embryonic stem (ES) cells proliferation and self-renewal. Imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. Blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes By similarity. Acts as a transcriptional activator or repressor By similarity. Binds optimally to the DNA consensus sequence 5'-TAAT[GT][GT]-3' or 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3' By similarity. When overexpressed, promotes cells to enter into S phase and proliferation.
• Synonyms: FLJ12581; FLJ40451; hNanog

Reference Data

• Protein Families: Cancer stem cells, Embryonic stem cells, ES Cell Differentiation/IPS, Induced pluripotent stem cells, Stem cell - Pluripotency