STAT3 Rabbit Polyclonal Antibody

CAT#: AP26348PU-N

STAT3 rabbit polyclonal antibody, Aff - Purified

Product Images

: Western blot analysis of Stat3 in lysate from Jurkat cells (Lane 1 & 2), 3T3 cells (Lane 3) and rat kidney (Lane 4).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot (0.5-4 µg/ml).
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 73 of rat Stat3
• Specificity: This antibody recognizes ~97 kDa Stat3.
• Formulation: Phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography
• Storage: Upon receipt, store at -20 °C. For long term storage, aliquot and store at -70 °C. Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: Homo sapiens signal transducer and activator of transcription 3 (acute-phase response factor) (STAT3), transcript variant 2
• Database Link:
  Entrez Gene 20848 MouseEntrez Gene 25125 RatEntrez Gene 6774 Human
• Background: Membrane receptor signaling by various ligands induces activation of Jak kinases which then leads to tyrosine phosphorylation of the various Stat transcription factors. Stat1 and Stat2 are induced by IFN-α and form a heterodimer which is part of the ISGF3 transcription factor complex. Although early reports indicate Stat3 activation by EGF and IL-6, it has been shown that Stat3β appears to be activated by both while Stat3α is activated by EGF, but not by IL-6. Highest expression of Stat4 is seen in testis and myeloid cells. IL-12 has been identified as an activator of Stat4. Stat5 is activated by prolactin and by IL-3. Stat6 is involved in IL-4 activated signaling pathways.
• Synonyms: STAT-3, Acute-phase response factor, APRF

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Chemokine signaling pathway, Jak-STAT signaling pathway, Pancreatic cancer, Pathways in cancer