TJP2 (411-467) Guinea Pig Polyclonal Antibody

CAT#: AP26409PU-N

TJP2 (411-467) guinea pig polyclonal antibody, Purified

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunohistochemistry on Frozen Sections.
  Immunofluorescence.
  Western blot.
  The typical starting working dilution is 1/50.
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Amino acids 411-467 of Human ZO-2
• Specificity: This antibody recognizes human zona occludens 2 (ZO-2).
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.1 mg/ml
• Purification: Protein A Chromatography
• Database Link:
  Entrez Gene 9414 Human
• Background:

  Zona occludens 2 (ZO-2) is an ~160 kDa tight junction protein belonging to the membrane-associated guanlyate kinase (MAGUK) family. Members of this family are involved in epithelial and endothelial intercellular junctions. They each contain at least one PSD95/Dlg/ZO-1 (PDZ) domain, a Src homology 3 (SH3) domain, and an enzymatically inactive guanylate kinase domain. PDZ domains are 90-amino acid protein-protein binding domains that recognize at least a 3-residue peptide motif in the COOH termini of their binding partners. PDZ domain-containing proteins, like ZO-2, typically act as scaffolding proteins that organize membrane receptors and cytosolic proteins into multimeric signaling complexes often at the sites of cell-cell contact. The effectiveness and stability of the epithelial barrier depends on a complex of proteins composing different intercellular junctions, which include tight junctions, adherens junctions, and desmosomes.
  ZO-2 can interact with zona occludens 1 (ZO-1). Furthermore, the PDZ2 domain of ZO-2 was shown to interact with connexin-43, the predominant connexin in epithelial and most other tissues which is involved in cell growth control and embryonic development.

• Synonyms: Tight junction protein 2, Tight junction protein ZO-2, ZO2, X104