SYN1 Rabbit Polyclonal Antibody

CAT#: AP26436PU-N

SYN1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot of 10 ug of rat hippocampal (Hipp) lysate showing specific immunolabeling of the ~78k synapsin I doublet protein.

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  Immunostaining of cultured rat caudate neurons showing punctate distribution of synapsin in green and MAP in red.

Specifications

Product Data

• Applications: IF, IP, WB
• Recommended Dilution: Western blot: 1/1000.
  Immunohistochemistry / immunoflourescence: 1:1000.
  Immunoprecipitation: 2 μl per 200 μg lysate.
• Reactivities: Bovine, Human, Mouse, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Native protein purified from bovine brain
• Specificity: Specific for the ~78k synapsin I doublet in Western blots of rat brain extracts. Immunolabeling blocked by preadsorption of antibody with the protein used to generate the antibody.
• Formulation: 100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg BSA per ml and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Purification: Affinity purification using a column to which the native protein was coupled
• Gene Name: -
• Database Link:
  Entrez Gene 281510 Bovine
• Background: Synapsin I plays a key role in synaptic plasticity in brain (Feng et al., 2002; Nayak et al., 1996). This effect is due in large part to the ability of the synapsins to regulate the availability of synaptic vesicles for release. In addition to its role in plasticity, the expression of synapsin I is a precise indicator of synapse formation (Moore and Bernstein, 1989; Stone et al., 1994). Thus, synapsin I immunocytochemistry provides a valuable tool for the study of synaptogenesis. The role of synapsin in synaptic plasticity and in synaptogensis is regulated by phosphorylation (Jovanovic et al., 2001; Kao et al., 2002).
• Synonyms: Synapsin I, Brain protein 4.1, SYN1