Tnni3 pSer150 Rabbit Polyclonal Antibody

CAT#: AP26441PU-N

Tnni3 pSer150 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot of mouse heart lysate showing specific imm unolabeling of ~25k cTnI protein phosphorylated at Ser 150 (control). Phosphospecificity is shown in the second lane (lambda -phosphatase: λ -Ptase). The blot is identical to the control except that the lysate was incubated in λ -Ptase (1400 units for 30 min). The immunolabeling is greatly decreased by treatment with λ -Ptase.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/1000. Immunolabeling is greatly decreased with lambda -phosphatase treatment.
• Reactivities: Human, Mouse, Primate, Rat
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser 150 of Mouse Troponin I, cardiac (cTnI).
• Specificity: Specific for the ~25k cardiac troponin I protein phosphorylated at Ser 150.
• Formulation: 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Purification: Affinity purification via sequential chromatography on phospho- and dephospho-peptide affinity columns
• Database Link:
  Entrez Gene 21954 Mouse
• Background: Troponin I (TnI) is 1 of 3 subunits, along with troponin C (TnC) and Troponin T (TnT) of troponin complex found in cardiac (cTnI) and fast skeletal (fsTnI) muscle. cTnI is phosphorylated by protein kinase C and protein kinase A at Ser 23/24 (Noland et al, 1995) and is phosphorylated by AMPK at Ser 23 and Ser 150 (Solis et al, 2011). Evidence su ggests that AMPK, a critical regulator of cardiac energetics, prefers phosphorylating Ser 150 over Ser 23 , and may play a role in regulating energy consumption through altering the p hosphorylation status of cTnI (Solis et al., 2011).
• Synonyms: TNNI3, TNNC1