Adenosine Receptor A2a (ADORA2A) (C-term) Goat Polyclonal Antibody

CAT#: AP31068PU-N

Adenosine Receptor A2a (ADORA2A) (C-term) goat polyclonal antibody, Aff - Purified

Product Images

AP31068PU-N Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Peptide ELISA: 1/8000 (Detection Limit).
  Western Blot: This antibody has been successfully used on lysates of primary Rhesus microglia (See Reference 1 for more details).
  Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the cytoplasm of HepG2 cells.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the C-Terminus of the protein sequence according to NP_000666.2.
• Specificity: This antibody detects Adenosine A2a Receptor.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 135 Human
• Background: The A2a Adenosine Receptor is a major target of caffeine. This receptor inhibits cell aggregation, induces vasodilation, and downregulates inflammation. This gene encodes a protein which is one of several receptor subtypes for adenosine. The activity of the encoded protein, a G protein coupled receptor family member, is mediated by G proteins which activate adenylyl cyclase. The encoded protein is abundant in basal ganglia, vasculature and platelets and it is a major target of caffeine.
• Synonyms: ADORA2A, ADORA2