Prolyl Endopeptidase (PREP) Goat Polyclonal Antibody

CAT#: AP31109PU-N

Prolyl Endopeptidase (PREP) goat polyclonal antibody, Aff - Purified

Product Images

PREP antibody staining of Paraffin Embedded Human Kidney at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/16000.
  Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 3-6 µg/ml.
  In Paraffin Embedded Human Kidney shows distinct staining patterns in the cytoplam of PCT.
• Reactivities: Bovine, Canine, Human, Mouse, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence (NP_002717.3).
• Formulation: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens prolyl endopeptidase (PREP)
• Database Link:
  Entrez Gene 19072 MouseEntrez Gene 83471 RatEntrez Gene 5550 Human
• Background: Prolyl Endopeptidase belongs to the peptidase S9A family.
  Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
• Synonyms: Prolyl endopeptidase
• Note: Calculated Molecular Weight: 80.7kDa (NP_002717.3)

Reference Data

• Protein Families: Druggable Genome, Protease