Prolyl Endopeptidase (PREP) Goat Polyclonal Antibody

CAT#: AP31109PU-N

Prolyl Endopeptidase (PREP) goat polyclonal antibody, Aff - Purified


USD 375.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications ELISA, IHC
Recommended Dilution Peptide ELISA: Detection Limit: 1/16000.
Western Blot: Preliminary experiments gave an approx 45kDa band in Human Brain, Lymph Node, Spleen and Testis lysates after 0.3 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 80.7kDa according to NP_002717.3. The 45kDa band was successfully blocked by incubation with the immunizing peptide.
Immunohistochemistry on Paraffin Sections: 3-6 µg/ml.
In Paraffin Embedded Human Kidney shows distinct staining patterns in the cytoplam of PCT.
Reactivities Bovine, Canine, Human, Mouse, Porcine, Rat
Host Goat
Clonality Polyclonal
Immunogen Peptide with sequence from the internal region of the protein sequence (NP_002717.3).
Formulation Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
State: Aff - Purified
State: Liquid purified Ig fraction
Concentration lot specific
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Gene Name Homo sapiens prolyl endopeptidase (PREP)
Background Prolyl Endopeptidase belongs to the peptidase S9A family.
Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long.
Synonyms Prolyl endopeptidase
Note Calculated Molecular Weight: 80.7kDa (NP_002717.3)
Reference Data
Protein Families Druggable Genome, Protease

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