PACE4 (PCSK6) Goat Polyclonal Antibody

CAT#: AP31111PU-N

PACE4 (PCSK6) goat polyclonal antibody, Aff - Purified

Product Images

PACE4 antibody staining of Paraffin Embedded Human Skeletal Muscle at 5 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/32000.
  Western Blot: Preliminary experiments gave an approx 40-45 kDa band in Human Brain (Cerebellum) lysates after 0.2 µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 106 kDa according to NP_002561.1. The 40-45 kDa band was successfully blocked by incubation with the immunizing peptide.
  Immunohistochemistry on Paraffin Sections: 5-10 µg/ml.
  In Paraffin Embedded Human Skeletal Muscle shows striated staining in alternating fibres.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002561.1; NP_612192.1; NP_612195.1; NP_612197.1; NP_612196.1; NP_612198.2; NP_612193.1; NP_612194.1.
• Specificity: Recognizes PCSK6 / PACE4.
  This antibody is expected to recognize reported isoforms a (NP_002561.1), b (NP_612192.1), g (NP_612193.1) and h (NP_612194.1).
• Formulation: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Gene Name: Homo sapiens proprotein convertase subtilisin/kexin type 6 (PCSK6), transcript variant 1
• Database Link:
  Entrez Gene 5046 Human
• Background: PACE4 belongs to the peptidase S8 family.
  Likely to represent an endoprotease activity within the constitutive secretory pathway, with unique restricted distribution in both neuroendocrine and non-neuroendocrine tissues and capable of cleavage at the RX(K/R)R consensus motif.
  Each PACE4 isoform exhibits a unique restricted distribution. Isoform PACE4A-I is expressed in heart, brain, placenta, lung, skeletal muscle, kidney, pancreas, but at comparatively higher levels in the liver. Isoform PACE4A-II is at least expressed in placenta. Isoform PACE4B was only found in the embryonic kidney cell line from which it was isolated. Isoform PACE4C and isoform PACE4D are expressed in placenta. Isoform PACE4E-I is expressed in cerebellum, placenta and pituitary. Isoform PACE4E-II is at least present in cerebellum.
• Synonyms: SPC4

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein