Grm5 (C-term) Rabbit Polyclonal Antibody

CAT#: AP31115PU-N

Grm5 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

mGluR5 antibody staining of Rat globus pallidus. Images courtesy of Dr. Joyce Besheer, UNC.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 0.1-0.5 µg/ml.
  ECL on Rat microsmoal preparation.
  Reacts with a band of ~132 kDa.
  Immunocytochemistry: 0.5 µg/ml (See Protocols for more details).
  Immunohistochemistry: 0.1-0.2 µg/ml (See Protocols for more details).
• Reactivities: Canine, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-terminus of Rat mGluR5
• Specificity: This antibody recognizes mGluR5 at C-term.
• Formulation: 0.02M Phosphate, 0.2M Sodium Chloride, pH 7.6
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Preservative: 0.09% Sodium Azide
• Concentration: lot specific
• Purification: Afiinity Chromatography
• Storage: Store the antibody undiluted (in aliquots) at -20°C
  Antibody may have become trapped in top of vial during shipping.
  Centrifugation of vial is recommended before opening.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: 6 months from despatch.
• Gene Name: Rattus norvegicus glutamate metabotropic receptor 5 (Grm5)
• Database Link:
  Entrez Gene 108071 MouseEntrez Gene 24418 Rat
• Background: Metabotropic glutamate receptors (mGluRs) are G-protein coupled receptors activated by glutamate. Based on sequence similarity, transduction mechanisms and agonist potencies, mGluRs are subdivided into three groups: mGluR1/mGluR5, mGluR2/mGluR3, and mGluR4/mGluR6/mGluR7/mGluR8. mGluRs are widely distributed throughout the nervous system and are expressed by both neurons and glial cells. mGluRs have been suggested to play a variety of functional roles, among which is involvement in synaptic plasticity underlying learning and memory as well as chronic pain.
• Synonyms: Metabotropic glutamate receptor 5, GPRC1E, MGLUR5
• Note: Protocol: Immunohistochemistry: Rat brain and mouse brain sections fixed with 4% paraformaldehyde containing 15% saturated picric acid in 0.1M PB, pH 7.4.
  Slide-mounted tissue sections were processed for indirect Immunofluorescence.
  Slides were incubated with blocking buffer for 1 hour at room temperature.
  Primary antiserum was diluted with blocking buffer to the appropriate working concentration.
  Blocking buffer was removed and slides were incubated for 18-24 hours at 4ºC with primary antiserum.
  Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature.
  Slides were again rinsed 3 times and coverslipped.
  Staining was examined using Fluorescence microscopy.