beta III Tubulin (TUBB3) Chicken Polyclonal Antibody

CAT#: AP31148PU-N

beta III Tubulin (TUBB3) chicken polyclonal antibody, Aff - Purified

Product Images

Tuj1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Testis at 5 ug/ml after heat-induced antigen retrieval.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/250 - 1/500.
• Reactivities: Human, Mouse, Rat
• Host: Chicken
• Isotype: IgY
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region of the Tuj1 gene product shared between the Rat (AAM28438, NCBI) and Human (AAL28094, NCBI) gene products.
• Specificity: Recognizes Human Tuj1 (Neuron-specific class III beta-Tubulin).
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified IgY fraction
  Preservative: 0.02% Sodium Azide
• Concentration: 0.3 mg/ml
• Purification: Immunoaffinity Chromatography
• Database Link:
  Entrez Gene 10381 Human
• Background: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non exchangeable site on the alpha-chain. Tubulin is a highly conserved protein with a molecular weight of ~50 kD. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. The two main tubulin isoforms, α- and β-tubulin, are usually products of separate genes. The β-tubulin family includes six expressed genes that produce the polypeptide isoforms known as Classes I through VI, each of which have a distinct pattern of expression. Class III β-tubulin is found in neurons and mammalian testis cells and is widely used as a neuronal marker in developmental neurobiology, neoplasia, and stem cell research. Class III β-tubulin expression in neuronal and neuroblastic tumors is differentiation dependent, and its expression in certain non-neuronal neoplasms has been associated with poor prognosis and/or resistance to chemotherapy.
• Synonyms: Tubulin beta-3 chain, Tubulin beta-III, Tubulin beta-4