eNOS (NOS3) Rabbit Polyclonal Antibody

CAT#: AP31282PU-N

eNOS (NOS3) rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of eNOS in Jurkat Cell Lysate using eNOS antibody.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blotting (0.5-4 μg/ml).
• Reactivities: Bovine, Equine, Human, Monkey, Mouse, Porcine, Rat, Sheep
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 602 of Rat eNOS
• Specificity: The antibody recognizes 140 kDa eNOS.
• Formulation: PBS containing 30% Glycerol, 0.5% BSA, and 0.01% Thimerosal.
  State: Aff - Purified
  State: Liquid purified IgG fraction
• Concentration: lot specific
• Purification: Affinity Chromatography.
• Storage: Store the antibody at -20°C.
  For long term storage aliquot and freeze at -70°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nitric oxide synthase 3 (NOS3), transcript variant 1
• Database Link:
  Entrez Gene 18127 MouseEntrez Gene 24600 RatEntrez Gene 4846 Human
• Background: eNOS (Endothelial nitric-oxide synthase) is an important enzyme in the cardiovascular system. eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including
  Akt/PKB, PKA and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli. In contrast, bradykinin and hydrogen peroxide activate eNOS activity by promoting Thr495 dephosphorylation.
• Synonyms: Endothelial NOS, EC-NOS, NOS type III, Constitutive NOS, eNOS

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer, VEGF signaling pathway