CRMP2 (DPYSL2) (C-term) Rabbit Polyclonal Antibody

CAT#: AP31330PU-N

CRMP2 (DPYSL2) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from A549 cells, treated with EGF 200ng/ml 30', using DPYSL2 antibody. The lane on the right is treated with the synthesized peptide.

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  Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Colon, Peripheral Nerve: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Pancreas, Peripheral Nerve: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of HeLa cells, using DPYSL2 Antibody. The picture on the right is treated with the synthesized peptide.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/1000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated
• Specificity: This antibody detects endogenous levels of total DPYSL2 protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens dihydropyrimidinase-like 2 (DPYSL2), transcript variant 1
• Database Link:
  Entrez Gene 12934 MouseEntrez Gene 25416 RatEntrez Gene 1808 Human
• Background: DRP-2, also known as Collapsin Response Mediator Protein-2 (CRMP-2), is expressed at high levels in the developing nervous system and plays a critical role in axonal outgrowth by specifying axon/dendrite fate and establishing neuronal polarity. CRMP-2 enhances axon elongation and branching by binding to tubulin heterodimers to promote microtubule assembly. GSK-3b inactivates CRMP-2 by phosphorylating it at Thr514. CRMP-2 is primed following phosphorylation at Ser522 by CDK5 and at Thr518 by GSK-3b. Phosphorylation of CRMP-2, which decreases tubulin binding ability, can be inhibited by NT-3 and BDNF through the PI3 kinase/Akt pathway. CRMP-2 also mediates semaphorin-induced growth cone collapse. Hyperphosphorylation of CRMP-2 and is found in Alzheimer disease plaques with concurrent elevated GSK-3b activity in these patients.
• Synonyms: Dihydropyrimidinase-related protein 2, DRP-2, N2A3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance