Vinculin (VCL) Rabbit Polyclonal Antibody

CAT#: AP31334PU-N

Vinculin (VCL) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from HeLa cells, treated with forskolin 40nM 30 minutes, using Vinculin Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Kidney, Tubules: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of A549 cells, using Vinculin Antibody. The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/10000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated
• Specificity: This antibody detects endogenous levels of total Vinculin protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens vinculin (VCL), transcript variant 2
• Database Link:
  Entrez Gene 22330 MouseEntrez Gene 305679 RatEntrez Gene 7414 Human
• Background: Vinculin is a cytoskeletal protein associated with cell-cell and cell-matrix junctions, where it is thought to function as one of several interacting proteins involved in anchoring F-actin to the membrane. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. Human vinculin protein exhibits a greater than 95% sequence identity to the chicken vinculin protein.
• Synonyms: Metavinculin

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Adherens junction, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton