RHOH (C-term) Rabbit Polyclonal Antibody

CAT#: AP31335PU-N

RHOH (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from HT-29 cells, using RhoH Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of A549 cells, using RhoH Antibody. The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/5000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated
• Specificity: This antibody detects endogenous levels of total RhoH protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens ras homolog family member H (RHOH), transcript variant 6
• Database Link:
  Entrez Gene 74734 MouseEntrez Gene 305341 RatEntrez Gene 399 Human
• Background: The Rho subfamily of small GTP-binding proteins mediates many fundamental cellular functions. The commonly studied members (Rho, Rac, and CDC42) regulate actin reorganization and affect diverse cellular responses, including adhesion, cytokinesis, and motility. RhoH, also known as TTF (Translocation Three Four), Rho-related GTP-binding protein and ras homolog gene family member H, is unlike most other small G proteins. Most small G proteins are expressed ubiquitously, however, Rho H is expressed only in hemopoietic cells and tissues. Translocations and a high frequency of Rho H mutation have been detected in primary lymphoma cells. Rho H expression has also been observed in activated neutrophils. RhoH is GTPase deficient, remaining in a GTP-bound activated state without cycling. Rho H may be involved in the functional differentiation of T cells and in cytoskeleton organization. The RhoH/TTF (ARHH) gene maps to chromosome 4p13 and encodes a 191 -amino acid polypeptide.
• Synonyms: ARHH, TTF

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Leukocyte transendothelial migration