HGS Rabbit Polyclonal Antibody

CAT#: AP31347PU-N

HGS rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from 293/HuvEc cells, using HGS Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Pancreas, Islets of Langerhans: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of HuvEc cells, using HGS Antibody. The picture on the right is treated with the synthesized peptide.

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Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/40000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/100.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated
• Specificity: This antibody detects endogenous levels of total HGS protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4 containing 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: 1.0 mg/ml
• Purification: Immunoaffinity Chromatography.
• Gene Name: Homo sapiens hepatocyte growth factor-regulated tyrosine kinase substrate (HGS)
• Database Link:
  Entrez Gene 15239 MouseEntrez Gene 56084 RatEntrez Gene 9146 Human
• Background: HGS is involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. It could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. HGS may concentrate ubiquitinated receptors within clathrin-coated regions. It is involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM. This complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. HGS may contribute to the efficient recruitment of SMADs to the activin receptor complex.
• Synonyms: Protein pp110

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis