BMX (N-term) Rabbit Polyclonal Antibody

CAT#: AP31357PU-N

BMX (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from COS7 cells, using BMX Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Intestine, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE).

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  Human Placenta, Vessels: Formalin-Fixed, Paraffin-Embedded (FFPE).

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  Immunofluorescence analysis of A549 cells, using BMX Antibody. The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/20000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/50.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide - KLH conjugated
• Specificity: This antibody detects endogenous levels of total BMX protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol.
  State: Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens BMX non-receptor tyrosine kinase (BMX), transcript variant 2
• Database Link:
  Entrez Gene 12169 MouseEntrez Gene 660 Human
• Background: BMX/ETK, a BTK/TEC type kinase, has been shown to affect the regulation of cellular processes including proliferation, differentiation, motility, apoptosis, and endocytosis. Etk contains an N-terminal Pleckstrin homology (PH) domain, a src homology 3 (SH3) domain, a src homology 2 (SH2) domain and a C-terminal tyrosine kinase (SH1) domain. The PH domains of Btk type kinases have been shown to bind to phospholipids as well as many protein partners including heterotrimeric G-proteins, PKC isoforms, Stat3, F-actin, Fas and FAK. Etk is activated by il-6 through phosphatidylinositol 3-kinase (pi3-kinase) pathway. It is likely that activation occurs through binding of phosphoinositides to the ph domain. It has been shown that Pak1 is a target of Etk and that Etk controls the proliferation as well as the anchorage-independent and tumorigenic growth of mammary epithelial cancer cells.
• Synonyms: NTK38, ETK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase