C1QA Rabbit Polyclonal Antibody

CAT#: AP31523SU-N

C1QA rabbit polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in precipitating techniques as Immunoelectrophoresis and single or double radial Immunodiffusion to identify the presence of C1q or C1q containing complexes in Human plasma or other body fluids or to determine its concentration. The presence of non precipitating antibodies has not been assayed. This does not exclude the use of the antiserum in more sensitive non-precipitating antibody-binding techniques if proper controls are included.
  Antibody titre: Precipitin titre 1/32 when tested against in agar-block immunodiffusion titration against an
  appropriate reference.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The subunit C1q is isolated as a homogenous protein for use in antiserum production.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to C1q. In immunoelectrophoresis and radial immunodiffusion (Ouchterlony), using various antiserum concentrations against fresh normal human plasma a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified C1q protein. Complexes containing C1q may also react with the antiserum. No reaction is obtained with C1r, C1s or any other plasma protein component or serum.
  Cross-reactivity: The antiserum does not cross react with any other component of human plasma. Inter-species cross-reactivity is a normal feature of antibodies to plasma proteins since they frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized, Delipidated, Heat inactivated, stable whole antiserum
  Preservative: None
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Concentration: lot specific
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens complement component 1, q subcomponent, A chain (C1QA)
• Database Link:
  Entrez Gene 712 Human
• Background: C1 component of Human complement is a complex of three glycoproteins: C1q, C1r and C1s. C1q has a molecular weight of 492.000 and is present in plasma in an average concentration of 80 μg/ml. It consists of a complex of 18 polypeptide chains of three different types, A, B and C; 6 of each type. The subcomponent C1q is responsible for the binding of C1q to immunocomplexes an membranes via the Fc portion of the immunoglobulin.
• Synonyms: Complement C1q, Complement 1q
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other plasma proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reference Data

• Protein Families: Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus