Alb Goat Polyclonal Antibody

CAT#: AP31547SU-N

Alb goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP, R
• Recommended Dilution: Can be used as precipitating antiserum to identify or measure rat albumin by a variety of immunodiffusion techniques, including immunoelectrophoresis, single and double radial immunodiffusion (Mancini, Ouchterlony) and electroimmunodiffusion (Laurell). It has not been tested for use in nephelometry, ELISA or immunochemistry, but this does not exclude such use if proper controls are included.
  Directions for use:
  In immunoelectrophoresis use 2 μl serum, plasma or equivalent against 120 μl antiserum.
  In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μl antiserum in a 3 mm diameter centre well and 2 μl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  In single radial immunodiffusion and electroimmunodiffusion use 0.5 to 1.0 percent antiserum in the agar gel.
• Reactivities: Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Highly purified albumin isolated from rat serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The defined antibody specificity is directed to albumin as tested against rat sera. In immunoelectrophoresis and double radial immunodiffusion (Ouchterlony), using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against rat serum and the purified albumin.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to mammalian serum proteins, since homologous proteins of different species frequently share antigenic determinants. The degree of cross-reactivity is also dependent on the concentrations of the reactants and the sensitivity of the assay arrangement. This antiserum fraction has been tested for cross-reactivity by double radial immunodiffusion against several species sera with the following results:
  - cow, chicken, goat and sheep
  + dog, horse, human, monkey and swine
  ++ guinea pig and mouse
  ± rabbit
• Formulation: No preservative added - No foreign proteins added
  State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum
• Reconstitution Method: Restore with 1 ml sterile distilled water
• Gene Name: Rattus norvegicus albumin (Alb)
• Database Link:
  Entrez Gene 24186 Rat
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.