Grp75 (HSPA9) (C-term) Rabbit Polyclonal Antibody

CAT#: AP31621PU-N

Grp75 (HSPA9) (C-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of extracts from COS7 cells, using GRP75 Antibody. The lane on the right is treated with the synthesized peptide.

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  Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

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  Immunofluorescence analysis of COS7 cells, using GRP75 Antibody. The picture on the right is treated with the synthesized peptide.

Specifications

Product Data

• Applications: ELISA, IF, IHC, WB
• Recommended Dilution: ELISA: 1/5000.
  Immunofluorescence: 1/100 - 1/500.
  Immunohistochemistry on Paraffin Sections: 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to C-Terminus of Human GRP75.
• Specificity: This antibody detects endogenous levels of total GRP75 protein.
• Formulation: PBS (without Mg2+, Ca2+), pH 7.4, 150 mM Sodium Chloride, 0.02% Sodium Azide and 50% Glycerol
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody (in aliquots) at -20°C or lower.
  Avoid freeze-thaw cycles.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens heat shock 70kDa protein 9 (mortalin) (HSPA9)
• Database Link:
  Entrez Gene 15526 MouseEntrez Gene 291671 RatEntrez Gene 3313 Human
• Background: Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat inducible (1, 2). Grp75 is actually induced under conditions of low glucose and other nutritional and environmental stresses. Grp75 resides primarily in the mitochondrial matrix, where it collaborates with Hsp60 in the re-folding of proteins translocated into this organelle (3, 4). Related forms may also be found in the cytosol or on the surface of the extracellular membrane.
  Other Grp75 functions include its ability to inactivate the tumor suppressor p53 (5). Studies have found that Grp75 is over-expressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (6). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (7). And finally, like its E. coli homolog DnaK (8), GRP75 possesses a cation-dependent ATPase activity considered central to its function as a chaperone (9, 10).
• Synonyms: Heat shock 70 kDa protein 9, Peptide-binding protein 74, PBP74, MOT, HSPA9B, Stress-70 protein, Mortalin, 75 kDa glucose-regulated protein

Reference Data

• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation