Glycophorin A (GYPA) Rabbit Polyclonal Antibody

CAT#: AP31671PU-N

Glycophorin A (GYPA) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis using (Purified Anti-Glycophorin A pAb) at a dilution of 1/100 on human RBCs at various concentrations (GPA = Glycophorin A, GPB = Glycophorin B).

Specifications

Product Data

• Applications: ELISA, FC, WB
• Recommended Dilution: Flow Cytometry.
  Western Blot.
  ELISA.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human Glycophorin A transmembrane protein
  Donor: Rabbit serum
• Specificity: Anti-human Glycophorin A (GpA) Polyclonal Antibody targets the human Glycophorin A protein. This polyclonal antibody recognizes various epitopes on the GpA protein.
  The 23 residue hydrophobic transmembrane domain has been shown to mediate non-covalent dimerization of the protein under conditions of SDS page (and in other detergents).
  We also sell a FITC conjugated version of this antibody (AP31671FC-N).
• Formulation: PBS containing 0.02% sodium azide (NaN3) as preservative
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Affinity chromatography on Protein G
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens glycophorin A (MNS blood group) (GYPA), transcript variant 1
• Database Link:
  Entrez Gene 2993 Human
• Background: This 131 amino acid, 36 KDa, transmembrane sialoglycoprotein spans the membrane of erythrocytes once. GpA presents its N terminus at the extracellular surface of the human red blood cell, has a single transmembrane domain and an internal cytoplasmic C terminus. GpA contributes to the expression of blood group antigen M and N specificities, which work to prevent red blood cell aggregation in circulation.
  Studies show that GpA is expressed early on the cell surface during erythropoiesis as pluripotent stem cells are recruited to differentiate into erythrocytes. This makes GpA a useful marker for studying the developmental biology of hematopoietic progenitor cells.
• Synonyms: Glycophorin-A, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Hematopoietic cell lineage