pan Cytokeratin Guinea Pig Polyclonal Antibody

CAT#: AP31680SU-N

pan Cytokeratin guinea pig polyclonal antibody, Serum

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunoblotting (Western blot): 1/1,000 (ECL).
  Immunofluorescence Microscopy on Frozen Sections.
  Immunohistochemistry on Frozen Sections: 1/100.
  Immunohistochemistry on Paraffin Embedded Sections: 1/50 (after microwave pretreatment).
  Incubation Time: 1 h at RT.
  Note: For enhancement of staining preincubate fixed sections with 0.1 Triton X-100 (in PBS) for 1–5 min prior to first antibody incubation step.
• Reactivities: Human, Mouse
• Host: Guinea Pig
• Clonality: Polyclonal
• Immunogen: Synthetic peptides common to Human type I (acidic) and type II (basic) hair (trichocytic) Keratins K31-K40 (former designation hHa1-hHa8, Ka35 and Ka36): LESEDCKLPSNP-C; and K81-K86 (former designation hHb1-hHb4): VNVCVSSSRGGVV-C, both coupled to KLH
• Specificity: The antiserum stains most prominently Human hair Keratins K81-K86 (type II) and considerably weaker K31-K40 (type I) hair Keratins present in the hair cortex, hair cuticle and tumors derived therefrom.
• Formulation: State: Serum
  State: Liquid Stabilized Antiserum
  Preservative: 0.09% Sodium Azide
• Background: Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
• Synonyms: pan Keratin, Cytokeratin pan-reactive