Pgf Rabbit Polyclonal Antibody

CAT#: AP31725PU-N

Pgf rabbit polyclonal antibody, Purified

Product Images

Immunohistochemical staining of PlGF in paraffin-embedded mouse placenta embryo (a and b ) showing intense cytoplasmic staining in mouse placenta at E9. In the embryo a positive signal was observed in endothelial structures of highly vascularized organs. Cross-reactivity of antibody was disproved as staining of human placenta did not reveal any signal©. Lower panel shows higher magnification of boxes in a-c. The experimentswereperformedbyDr. Frank Bicker fromtheresearchgroup ??Molecular Signal Transduction?? (Prof. Dr. Mirko HH Schmidt), Institute ofMicroscopicAnatomyandNeurobiology, University Medical Center of Johannes Gutenberg University Mainz, Germany.

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  Immunofluorescence staining (green) of mouse lung tissue (ED18) with anti-Mouse PlGF Antibody. The experiment was performedbytheresearchgroupof Prof. Dr. J. Wilting, University Göttingen, Germany.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 2-5 μg/ml.
  No cross-reactivity with recombinant Human PlGF under non-reducing conditions is observed.
  Immunofluorescence/Immunohistochemistry: 1/200.
  Immunohistochemistry on Paraffin Sections.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (> 95%) recombinant Mouse PlGF (Ala24-Pro158) derived from Sf9 insect cells (Cat.-No AR26011PU-L).
• Specificity: Recognizes Mouse PlGF. Other species not tested.
• Formulation: 5mM PBS, pH 7.2
  Endotoxin level: < 0.1 EU/1 µg of the antibody (LAL)
  State: Purified
  State: Liquid purified IgG fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein A Chromatography
• Gene Name: Mus musculus placental growth factor (Pgf), transcript variant 1
• Database Link:
  Entrez Gene 18654 Mouse
• Background: Placenta growth factor (PlGF) is a member of the vascular endothelial growth factor (VEGF) family of growth factors. PlGF and VEGF share primary structural as well as limited amino acid sequence homology with the A and B chains of PDGF. All eight cysteine residues involved in intra and interchain disulfides are conserved among these growth factors. As a result of alternative splicing, three PlGF RNAs encoding monomeric human PlGF1, PlGF2 and PlGF3 isoform precursors containing 149, 179 and 219 amino acid residues, respectively, have been described. In normal mouse tissues, only one mouse PlGF mRNA encoding the equivalent of human PlGF2 has been identified. Mouse PlGF shares 65% amino acid identity with human PlGF2. The gene for PlGF has been mapped to mouse chromosome 12 and human chromosome 14. PlGF binds with high affinity to Flt1, but not to Flk1/KDR.
• Synonyms: PGFL, PLGF, PlGF