TIE2 (TEK) Rabbit Polyclonal Antibody

CAT#: AP31727PU-N

TIE2 (TEK) rabbit polyclonal antibody, Purified

Product Images

Western analysis of recombinant Human and Mouse sTIE-1 and sTIE-2 using a Polyclonal antibody directed against Human recombinant sTIE-2. There is a strong cross reactivity with Mouse sTIE-2 but not with Human and Mouse sTIE-1 visible.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 2-5 μg/ml.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Highly pure (>95%) recombinant Human soluble TIE-2 (Thr19-Lys745) derived from Insect cells.
• Specificity: Recognizes TIE-2 (CD202b/TEK)
• Formulation: PBS, pH 7.2
  State: Purified
  State: Liquid purified IgG fraction
• Reconstitution Method: Centrifuge vial prior to opening.
  Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Protein A Chromatography
• Storage: The lyophilized antibody is stable at RT for up to 1 month. The reconstituted antibody is stable for at least two weeks at 2-8°C. Frozen aliquots are stable for at least 6 months when stored at -20°C.
  Avoid repeated freeze-thaw cycles!
• Gene Name: Homo sapiens TEK receptor tyrosine kinase (TEK), transcript variant 1
• Database Link:
  Entrez Gene 21687 MouseEntrez Gene 7010 Human
• Background: TIE-1 (tyrosine kinase with Ig and EGF homology domains 1) and TIE-2/TEK comprise a receptor tyrosine kinase (RTK) subfamily with unique structural characteristics: two immunoglobulin-like domains flanking three epidermal growth factor (EGF)-like domains and followed by three fibronectin type III-like repeats in the extracellular region and a split tyrosine kinase domain in the cytoplasmic region.
  These receptors are expressed primarily on endothelial and hematopoietic progenitor cells and play critical roles in angiogenesis, vasculogenesis and hematopoiesis. Human TIE-2 cDNA encodes a 1124 amino acid (aa) residue precursor protein with an 18 residue putative signal peptide, a 727 residue extracellular domain and a 354 residue cytoplasmic domain. Two ligands, angiopoietin-1 (Ang1) and angiopoietin-2 (Ang2), which bind TIE-2 with high affinity have been identified. Ang2 has been reported to act as an antagonist for Ang1. Mice engineered to overexpress Ang2 or to lack Ang1 or TIE-2 display similar angiogenic defects.
• Synonyms: TIE2, TIE-2, Angiopoietin-1 receptor, p140 TEK

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane